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PMID: 16213894

These peptides work at concentrations where pH disruption can meaningfully reduce efficacy

0.3 ml aliquots of the supernatant, 0.75 ml of 150 mM phosphate buffer (pH 7.4) containing 5 mM EDTA, and 0.15 ml of 10 mM DTT were added to determine total ascorbate

Despite these positive results, the transition from animal to human model did not give the same encouraging outcomes, since most of clinical trials failed (71, 72)

Urata Y, Honma S, Goto S, Todoroki S, Iida T, Cho S, et al

specifically, the end-product, GSH, accumulates in the cell and acts as an inhibitor of -glutamylcysteine ligase activity, thereby preventing the overproduction of GSH 41 (Figure 2)