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After purification, islets were cultured overnight at 37 C in a humidified atmosphere of 95% air and 5% CO 2 in RPMI 1640 medium supplemented with 10% FBS, 100 units/ml penicillin and 0.1 mg/ml streptomycin

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7), we emphasize that the ICV component served primarily as a preclinical proof-of-concept to validate direct CNS engagement for neural repair

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