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Beads were washed four times with IP wash buffer (50 mM TrisHCl (pH 7.4), 150 mM NaCl, 0.1% (vol/vol) IGEPAL CA-630), then thrice with IP wash buffer lacking IGEPAL CA-630

318 , F298F314 (2020)

GE Healthcare) at 4C

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NAPQI formation can be monitored indirectly through the presence of circulating paracetamol-protein adducts (PPA)