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Hub Bonding: The attachment point between the steel cannula and the plastic Luer hub is a common point of failure in low-quality brands

When working with an eukaryotic expression system that may contain unknown agents and factors interfering the binding of target protein, it is recommended to dilute the culture supernatant 1:1 or 1:2 with the Equilibration buffer (for example, 20 mM sodium phosphate, 0.5 M NaCl, pH 7.4)

Kusakabe J et al

Biotechnol Genet Eng Rev

Effective neuromuscular control relies on the rapid transmission of electrical signals from the brain to the muscles, mediated by motor neurons

The activation of ferroptosis requires transferrin receptors and transferrin to transport iron outside the cells