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The cells were subsequently treated with DCFH-DA (10 M, Bio-lab) for 1 h in the dark, and washed three times with PBS

International journal of cosmetic science , 22 (3), 207218

The enhanced cocktails, CILy (CHIR+IDE1+Ly) and CIP (CHIR+IDE1+PD), significantly increased the expression of DE markers SOX17 and FOXA2, with CIP showing the highest efficiency, comparable to the traditional CA method

NAD+ acts as the engine for energy metabolism, DNA repair, and longevity proteins