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After 72 h, the cells were lysed in 50 mM triethylammonium bicarbonate + 5% SDS buffer supplemented with Halt TM Protease Inhibitor Cocktail (Thermo Fisher Scientific)

Most TB500 protocols follow a structured 68 week cycle, broken into a loading section followed by a maintenance section

johnsonii , is decreased in late-stage CKD patients and in CKD rats induced with 5/6 nephrectomy (NX) or unilateral ureteral obstruction (UUO), showing a negative correlation with serum creatinine ( Table 1 ) [106]

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Release 345 , 755769 (2022)