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VEGF mRNA expression was quantitated in five sections each obtained from three mice for each time point by counting individual photographic grains over follicular keratinocytes as described (19), and biopsies were scored as negative (01 grains per cell

doi: 10.1038/ijo.2008.202 46 ManciniADi SegniC

Published animal research uses 10 g/kg daily the allometric human equivalent is roughly 200300 g/day

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Other investigators have attempted to facilitate the availability of central brain-derived neurotrophic factor (BDNF) with injections of human BDNF directly into the patients brain with minimal positive results [17]